Development of vaccines and passive immunotherapy against SARS coronavirus using mouse and SCID-PBL/hu mouse models.
Identifieur interne : 003F23 ( Main/Exploration ); précédent : 003F22; suivant : 003F24Development of vaccines and passive immunotherapy against SARS coronavirus using mouse and SCID-PBL/hu mouse models.
Auteurs : Masaji Okada [Japon] ; Yuji Takemoto ; Yoshinobu Okuno ; Satomi Hashimoto ; Yukari Fukunaga ; Takao Tanaka ; Yoko Kita ; Sechiko Kuwayama ; Yumiko Muraki ; Noriko Kanamaru ; Hiroko Takai ; Chika Okada ; Yayoi Sakaguchi ; Izumi Furukawa ; Kyoko Yamada ; Miwa Izumiya ; Shigeto Yoshida ; Makoto Matsumoto ; Tetsuo Kase ; J S M. Peiris ; Daphne E. Demello ; Pei-Jer Chen ; Naoki Yamamoto ; Yoshiyuki Yoshinaka ; Tatsuji Nomura ; Isao Ishida ; Shigeru Morikawa ; Masato Tashiro ; Mitsunori SakataniSource :
- Advances in experimental medicine and biology [ 0065-2598 ] ; 2006.
Descripteurs français
- KwdFr :
- Animaux, Humains, Immunisation passive (), Modèles animaux de maladie humaine, Souris, Souris SCID, Souris de lignée BALB C, Souris de lignée C57BL, Souris transgéniques, Syndrome respiratoire aigu sévère (), Techniques de coculture, Vaccins antiviraux, Virus du SRAS (génétique), Virus du SRAS (métabolisme).
- MESH :
- génétique : Virus du SRAS.
- métabolisme : Virus du SRAS.
- Animaux, Humains, Immunisation passive, Modèles animaux de maladie humaine, Souris, Souris SCID, Souris de lignée BALB C, Souris de lignée C57BL, Souris transgéniques, Syndrome respiratoire aigu sévère, Techniques de coculture, Vaccins antiviraux.
English descriptors
- KwdEn :
- MESH :
- chemical : Viral Vaccines.
- genetics : SARS Virus.
- metabolism : SARS Virus.
- methods : Immunization, Passive.
- prevention & control : Severe Acute Respiratory Syndrome.
- Animals, Coculture Techniques, Disease Models, Animal, Humans, Mice, Mice, Inbred BALB C, Mice, Inbred C57BL, Mice, SCID, Mice, Transgenic.
Abstract
We have investigated novel vaccines strategies against severe acute respiratory syndrome (SARS) CoV infection using cDNA constructs encoding the structural antigens; spike (S), membrane (M), envelope (E), or nucleocapsid (N) protein, derived from SARS CoV (strain HKU39849, TW1, or FFM-1). As SARS-CoV is thought to infect the alveolar epithelial cell of the lung,in the present study, a type II alveolar epithelial cell clone, T7, was used to analyze the mechanism of CTL against SARS CoV membrane antigens. Mice vaccinated with SARS CoV (N) DNA or (M) DNA using pcDNA 3.1 (+) plasmid vector showed T-cell immune responses (CTL induction and proliferation) against type II alveolar epithelial cells (T7) transfected with SARS (N) or (M) DNA, respectively. To determine whether these DNA vaccines could induce T-cell immune responses in humans as well as in mice, SCID-PBL/hu mice were immunized with these DNA vaccines. PBL from healthy human volunteers were administered i.p. into IL-2 receptor gamma-chain-disrupted NOD-SCID mice [IL-2R(-/-) NOD-SCID]. SCID-PBL/hu mice thus constructed can be used to analyze the human immune response in vivo. The SCID-PBL/hu mice were immunized with SARS (N) DNA or (M) DNA and analyzed for a human T-cell immune response. The M DNA vaccine enhanced CTL activity and proliferation in the presence of M peptide in SCID-PBL/hu mice. Furthermore, the SARS N DNA vaccine induced CTL activity (IFN-gamma production by recombinant N protein or N protein-pulsed autologous B blast cells) and proliferation of spleen cells in SCID-PBL/hu mice. These results, demonstrate that SARS M and N DNA vaccines induced human CTL and human T-cell proliferative responses. On the other hand, we have developed SARS DNA vaccines that induce human neutralizing antibodies and human monoclonal antibodies against SARS CoV. Transgenic mice expressing SARS-CoV receptor (angiotensin converting enzyme 2) are also under development. These vaccines are expected to induce immune responses specific for SARS CoV in human and should provide useful tool for development of protective vaccines.
DOI: 10.1007/978-0-387-33012-9_102
PubMed: 17037598
Affiliations:
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Osaka</wicri:regionArea><wicri:noRegion>Osaka</wicri:noRegion></affiliation></author><author><name sortKey="Takemoto, Yuji" sort="Takemoto, Yuji" uniqKey="Takemoto Y" first="Yuji" last="Takemoto">Yuji Takemoto</name></author><author><name sortKey="Okuno, Yoshinobu" sort="Okuno, Yoshinobu" uniqKey="Okuno Y" first="Yoshinobu" last="Okuno">Yoshinobu Okuno</name></author><author><name sortKey="Hashimoto, Satomi" sort="Hashimoto, Satomi" uniqKey="Hashimoto S" first="Satomi" last="Hashimoto">Satomi Hashimoto</name></author><author><name sortKey="Fukunaga, Yukari" sort="Fukunaga, Yukari" uniqKey="Fukunaga Y" first="Yukari" last="Fukunaga">Yukari Fukunaga</name></author><author><name sortKey="Tanaka, Takao" sort="Tanaka, Takao" uniqKey="Tanaka T" first="Takao" last="Tanaka">Takao Tanaka</name></author><author><name sortKey="Kita, Yoko" sort="Kita, Yoko" uniqKey="Kita Y" first="Yoko" last="Kita">Yoko Kita</name></author><author><name sortKey="Kuwayama, Sechiko" sort="Kuwayama, Sechiko" uniqKey="Kuwayama S" first="Sechiko" last="Kuwayama">Sechiko Kuwayama</name></author><author><name sortKey="Muraki, Yumiko" sort="Muraki, Yumiko" uniqKey="Muraki Y" first="Yumiko" last="Muraki">Yumiko Muraki</name></author><author><name sortKey="Kanamaru, Noriko" sort="Kanamaru, Noriko" uniqKey="Kanamaru N" first="Noriko" last="Kanamaru">Noriko Kanamaru</name></author><author><name sortKey="Takai, Hiroko" sort="Takai, Hiroko" uniqKey="Takai H" first="Hiroko" last="Takai">Hiroko Takai</name></author><author><name sortKey="Okada, Chika" sort="Okada, Chika" uniqKey="Okada C" first="Chika" last="Okada">Chika Okada</name></author><author><name sortKey="Sakaguchi, Yayoi" sort="Sakaguchi, Yayoi" uniqKey="Sakaguchi Y" first="Yayoi" last="Sakaguchi">Yayoi Sakaguchi</name></author><author><name sortKey="Furukawa, Izumi" sort="Furukawa, Izumi" uniqKey="Furukawa I" first="Izumi" last="Furukawa">Izumi Furukawa</name></author><author><name sortKey="Yamada, Kyoko" sort="Yamada, Kyoko" uniqKey="Yamada K" first="Kyoko" last="Yamada">Kyoko Yamada</name></author><author><name sortKey="Izumiya, Miwa" sort="Izumiya, Miwa" uniqKey="Izumiya M" first="Miwa" last="Izumiya">Miwa Izumiya</name></author><author><name sortKey="Yoshida, Shigeto" sort="Yoshida, Shigeto" uniqKey="Yoshida S" first="Shigeto" last="Yoshida">Shigeto Yoshida</name></author><author><name sortKey="Matsumoto, Makoto" sort="Matsumoto, Makoto" uniqKey="Matsumoto M" first="Makoto" last="Matsumoto">Makoto Matsumoto</name></author><author><name sortKey="Kase, Tetsuo" sort="Kase, Tetsuo" uniqKey="Kase T" first="Tetsuo" last="Kase">Tetsuo Kase</name></author><author><name sortKey="Peiris, J S M" sort="Peiris, J S M" uniqKey="Peiris J" first="J S M" last="Peiris">J S M. Peiris</name></author><author><name sortKey="Demello, Daphne E" sort="Demello, Daphne E" uniqKey="Demello D" first="Daphne E" last="Demello">Daphne E. Demello</name></author><author><name sortKey="Chen, Pei Jer" sort="Chen, Pei Jer" uniqKey="Chen P" first="Pei-Jer" last="Chen">Pei-Jer Chen</name></author><author><name sortKey="Yamamoto, Naoki" sort="Yamamoto, Naoki" uniqKey="Yamamoto N" first="Naoki" last="Yamamoto">Naoki Yamamoto</name></author><author><name sortKey="Yoshinaka, Yoshiyuki" sort="Yoshinaka, Yoshiyuki" uniqKey="Yoshinaka Y" first="Yoshiyuki" last="Yoshinaka">Yoshiyuki Yoshinaka</name></author><author><name sortKey="Nomura, Tatsuji" sort="Nomura, Tatsuji" uniqKey="Nomura T" first="Tatsuji" last="Nomura">Tatsuji Nomura</name></author><author><name sortKey="Ishida, Isao" sort="Ishida, Isao" uniqKey="Ishida I" first="Isao" last="Ishida">Isao Ishida</name></author><author><name sortKey="Morikawa, Shigeru" sort="Morikawa, Shigeru" uniqKey="Morikawa S" first="Shigeru" last="Morikawa">Shigeru Morikawa</name></author><author><name sortKey="Tashiro, Masato" sort="Tashiro, Masato" uniqKey="Tashiro M" first="Masato" last="Tashiro">Masato Tashiro</name></author><author><name sortKey="Sakatani, Mitsunori" sort="Sakatani, Mitsunori" uniqKey="Sakatani M" first="Mitsunori" last="Sakatani">Mitsunori Sakatani</name></author></analytic><series><title level="j">Advances in experimental medicine and biology</title><idno type="ISSN">0065-2598</idno><imprint><date when="2006" type="published">2006</date></imprint></series></biblStruct></sourceDesc></fileDesc><profileDesc><textClass><keywords scheme="KwdEn" xml:lang="en"><term>Animals</term><term>Coculture Techniques</term><term>Disease Models, Animal</term><term>Humans</term><term>Immunization, Passive (methods)</term><term>Mice</term><term>Mice, Inbred BALB C</term><term>Mice, Inbred C57BL</term><term>Mice, SCID</term><term>Mice, Transgenic</term><term>SARS Virus (genetics)</term><term>SARS Virus (metabolism)</term><term>Severe Acute Respiratory Syndrome (prevention & control)</term><term>Viral Vaccines</term></keywords><keywords scheme="KwdFr" xml:lang="fr"><term>Animaux</term><term>Humains</term><term>Immunisation passive ()</term><term>Modèles animaux de maladie humaine</term><term>Souris</term><term>Souris SCID</term><term>Souris de lignée BALB C</term><term>Souris de lignée C57BL</term><term>Souris transgéniques</term><term>Syndrome respiratoire aigu sévère ()</term><term>Techniques de coculture</term><term>Vaccins antiviraux</term><term>Virus du SRAS (génétique)</term><term>Virus du SRAS (métabolisme)</term></keywords><keywords scheme="MESH" type="chemical" xml:lang="en"><term>Viral Vaccines</term></keywords><keywords scheme="MESH" qualifier="genetics" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="génétique" xml:lang="fr"><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="metabolism" xml:lang="en"><term>SARS Virus</term></keywords><keywords scheme="MESH" qualifier="methods" xml:lang="en"><term>Immunization, Passive</term></keywords><keywords scheme="MESH" qualifier="métabolisme" xml:lang="fr"><term>Virus du SRAS</term></keywords><keywords scheme="MESH" qualifier="prevention & control" xml:lang="en"><term>Severe Acute Respiratory Syndrome</term></keywords><keywords scheme="MESH" xml:lang="en"><term>Animals</term><term>Coculture Techniques</term><term>Disease Models, Animal</term><term>Humans</term><term>Mice</term><term>Mice, Inbred BALB C</term><term>Mice, Inbred C57BL</term><term>Mice, SCID</term><term>Mice, Transgenic</term></keywords><keywords scheme="MESH" xml:lang="fr"><term>Animaux</term><term>Humains</term><term>Immunisation passive</term><term>Modèles animaux de maladie humaine</term><term>Souris</term><term>Souris SCID</term><term>Souris de lignée BALB C</term><term>Souris de lignée C57BL</term><term>Souris transgéniques</term><term>Syndrome respiratoire aigu sévère</term><term>Techniques de coculture</term><term>Vaccins antiviraux</term></keywords></textClass></profileDesc></teiHeader><front><div type="abstract" xml:lang="en">We have investigated novel vaccines strategies against severe acute respiratory syndrome (SARS) CoV infection using cDNA constructs encoding the structural antigens; spike (S), membrane (M), envelope (E), or nucleocapsid (N) protein, derived from SARS CoV (strain HKU39849, TW1, or FFM-1). As SARS-CoV is thought to infect the alveolar epithelial cell of the lung,in the present study, a type II alveolar epithelial cell clone, T7, was used to analyze the mechanism of CTL against SARS CoV membrane antigens. Mice vaccinated with SARS CoV (N) DNA or (M) DNA using pcDNA 3.1 (+) plasmid vector showed T-cell immune responses (CTL induction and proliferation) against type II alveolar epithelial cells (T7) transfected with SARS (N) or (M) DNA, respectively. To determine whether these DNA vaccines could induce T-cell immune responses in humans as well as in mice, SCID-PBL/hu mice were immunized with these DNA vaccines. PBL from healthy human volunteers were administered i.p. into IL-2 receptor gamma-chain-disrupted NOD-SCID mice [IL-2R(-/-) NOD-SCID]. SCID-PBL/hu mice thus constructed can be used to analyze the human immune response in vivo. The SCID-PBL/hu mice were immunized with SARS (N) DNA or (M) DNA and analyzed for a human T-cell immune response. The M DNA vaccine enhanced CTL activity and proliferation in the presence of M peptide in SCID-PBL/hu mice. Furthermore, the SARS N DNA vaccine induced CTL activity (IFN-gamma production by recombinant N protein or N protein-pulsed autologous B blast cells) and proliferation of spleen cells in SCID-PBL/hu mice. These results, demonstrate that SARS M and N DNA vaccines induced human CTL and human T-cell proliferative responses. On the other hand, we have developed SARS DNA vaccines that induce human neutralizing antibodies and human monoclonal antibodies against SARS CoV. Transgenic mice expressing SARS-CoV receptor (angiotensin converting enzyme 2) are also under development. These vaccines are expected to induce immune responses specific for SARS CoV in human and should provide useful tool for development of protective vaccines.</div></front></TEI><affiliations><list><country><li>Japon</li></country></list><tree><noCountry><name sortKey="Chen, Pei Jer" sort="Chen, Pei Jer" uniqKey="Chen P" first="Pei-Jer" last="Chen">Pei-Jer Chen</name><name sortKey="Demello, Daphne E" sort="Demello, Daphne E" uniqKey="Demello D" first="Daphne E" last="Demello">Daphne E. Demello</name><name sortKey="Fukunaga, Yukari" sort="Fukunaga, Yukari" uniqKey="Fukunaga Y" first="Yukari" last="Fukunaga">Yukari Fukunaga</name><name sortKey="Furukawa, Izumi" sort="Furukawa, Izumi" uniqKey="Furukawa I" first="Izumi" last="Furukawa">Izumi Furukawa</name><name sortKey="Hashimoto, Satomi" sort="Hashimoto, Satomi" uniqKey="Hashimoto S" first="Satomi" last="Hashimoto">Satomi Hashimoto</name><name sortKey="Ishida, Isao" sort="Ishida, Isao" uniqKey="Ishida I" first="Isao" last="Ishida">Isao Ishida</name><name sortKey="Izumiya, Miwa" sort="Izumiya, Miwa" uniqKey="Izumiya M" first="Miwa" last="Izumiya">Miwa Izumiya</name><name sortKey="Kanamaru, Noriko" sort="Kanamaru, Noriko" uniqKey="Kanamaru N" first="Noriko" last="Kanamaru">Noriko Kanamaru</name><name sortKey="Kase, Tetsuo" sort="Kase, Tetsuo" uniqKey="Kase T" first="Tetsuo" last="Kase">Tetsuo Kase</name><name sortKey="Kita, Yoko" sort="Kita, Yoko" uniqKey="Kita Y" first="Yoko" last="Kita">Yoko Kita</name><name sortKey="Kuwayama, Sechiko" sort="Kuwayama, Sechiko" uniqKey="Kuwayama S" first="Sechiko" last="Kuwayama">Sechiko Kuwayama</name><name sortKey="Matsumoto, Makoto" sort="Matsumoto, Makoto" uniqKey="Matsumoto M" first="Makoto" last="Matsumoto">Makoto Matsumoto</name><name sortKey="Morikawa, Shigeru" sort="Morikawa, Shigeru" uniqKey="Morikawa S" first="Shigeru" last="Morikawa">Shigeru Morikawa</name><name sortKey="Muraki, Yumiko" sort="Muraki, Yumiko" uniqKey="Muraki Y" first="Yumiko" last="Muraki">Yumiko Muraki</name><name sortKey="Nomura, Tatsuji" sort="Nomura, Tatsuji" uniqKey="Nomura T" first="Tatsuji" last="Nomura">Tatsuji Nomura</name><name sortKey="Okada, Chika" sort="Okada, Chika" uniqKey="Okada C" first="Chika" last="Okada">Chika Okada</name><name sortKey="Okuno, Yoshinobu" sort="Okuno, Yoshinobu" uniqKey="Okuno Y" first="Yoshinobu" last="Okuno">Yoshinobu Okuno</name><name sortKey="Peiris, J S M" sort="Peiris, J S M" uniqKey="Peiris J" first="J S M" last="Peiris">J S M. Peiris</name><name sortKey="Sakaguchi, Yayoi" sort="Sakaguchi, Yayoi" uniqKey="Sakaguchi Y" first="Yayoi" last="Sakaguchi">Yayoi Sakaguchi</name><name sortKey="Sakatani, Mitsunori" sort="Sakatani, Mitsunori" uniqKey="Sakatani M" first="Mitsunori" last="Sakatani">Mitsunori Sakatani</name><name sortKey="Takai, Hiroko" sort="Takai, Hiroko" uniqKey="Takai H" first="Hiroko" last="Takai">Hiroko Takai</name><name sortKey="Takemoto, Yuji" sort="Takemoto, Yuji" uniqKey="Takemoto Y" first="Yuji" last="Takemoto">Yuji Takemoto</name><name sortKey="Tanaka, Takao" sort="Tanaka, Takao" uniqKey="Tanaka T" first="Takao" last="Tanaka">Takao Tanaka</name><name sortKey="Tashiro, Masato" sort="Tashiro, Masato" uniqKey="Tashiro M" first="Masato" last="Tashiro">Masato Tashiro</name><name sortKey="Yamada, Kyoko" sort="Yamada, Kyoko" uniqKey="Yamada K" first="Kyoko" last="Yamada">Kyoko Yamada</name><name sortKey="Yamamoto, Naoki" sort="Yamamoto, Naoki" uniqKey="Yamamoto N" first="Naoki" last="Yamamoto">Naoki Yamamoto</name><name sortKey="Yoshida, Shigeto" sort="Yoshida, Shigeto" uniqKey="Yoshida S" first="Shigeto" last="Yoshida">Shigeto Yoshida</name><name sortKey="Yoshinaka, Yoshiyuki" sort="Yoshinaka, Yoshiyuki" uniqKey="Yoshinaka Y" first="Yoshiyuki" last="Yoshinaka">Yoshiyuki Yoshinaka</name></noCountry><country name="Japon"><noRegion><name sortKey="Okada, Masaji" sort="Okada, Masaji" uniqKey="Okada M" first="Masaji" last="Okada">Masaji Okada</name></noRegion></country></tree></affiliations></record>Pour manipuler ce document sous Unix (Dilib)
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|flux= Main
|étape= Exploration
|type= RBID
|clé= pubmed:17037598
|texte= Development of vaccines and passive immunotherapy against SARS coronavirus using mouse and SCID-PBL/hu mouse models.
}}
Pour générer des pages wiki
HfdIndexSelect -h $EXPLOR_AREA/Data/Main/Exploration/RBID.i -Sk "pubmed:17037598" \
| HfdSelect -Kh $EXPLOR_AREA/Data/Main/Exploration/biblio.hfd \
| NlmPubMed2Wicri -a SrasV1
| This area was generated with Dilib version V0.6.33. |  |